TEST METHOD AND VALUATION
EN 1276 chemical disinfectants and antiseptics-
Quantitative suspension test for the evaluation of bactericidal
activity of chemical disinfectants and antiseptics used in food,
industrial, domestic and institutional areas (Phase 2, step 1).
REQUIREMENT
The test product when tested in accordance
with the test methodology described under simulated clean and
dirty conditions shall demonstrate at least a 5 
reduction.
SUMMARY
OF TEST METHODS
EN 1276 Chemical disinfectants and antiseptics
- Quantitative suspension test for the evaluation of bactericidal
activity of chemical disinfectants and antiseptics used in food
, industrial, domestic and institutional areas (Phase 2, step
1).
Copies available from: BSI, 389 Chiswick High
Road, London W4 4AL
The test method involves mixing 1 ml of the test bacteria n with
1 ml of soil (0.3 or 3% albumin and then adding 8 ml of disinfectant.
After the required contact time, 1 ml is removed to 9 ml of recovery/neutralizer,
which is then plated to detect surviving test bacteria.
RESULTS BACTERCIDAL ACTIVITY OF
D-Stroy/Activ8 USING PHASE 2 STEP 1 SUSPENSION TEST
EN 1276
Log 10 counts/reductions achieved in 1, 2 and 5 minutes
(Tests carried out in duplicate)
*A contact time of 5 mins is statede
in EN 1276. However 1, 2 and 5 mins contact times were commissioned
as more relevant for hard surface for use in health care premises.
The tests carried out were therefore, more stringent.
CONCLUSION
When tested in accordance with EN 1276 (1997).
Undiluted D-Stroy/Activ8 solution has activity against MRSA at
20°C A>5
(99.999%) reduction was achieved with MRSA in 1 min, 2 mins and
5 mins under clean (0.03% albumin) and dirty (0.3% albumin) conditions.
To satisfy the requirements for the test at least a 5
reduction in specified test organisms is required with 5 mins
when the disinfectant is tested at its intended use dilution(s).
D-Stroy/Activ8, therefore, satisfies the requirements of the test
using MRSA as the test organism.
Performance under light (clean) and moderate to heavy (dirty)
soiling was assessed. The presence of soil does not appear to
affect the performance of the product.
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